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A gene is regulated by a novel transcription factor. The following techniques may be used to identify the cis – regulatory element in the 1 KB promoter sequence of the gene where the novel transcription factor binds:1. Restriction endonuclease assay2. Cell based reporter assay3. S1 nuclease assay 4. Electrophoretic mobility shift assay5. DNAse – 1 foot printing analysis.Which one of the following can help to identify the cis element?(A)1 and 2 (B) 3 and 5(C) Only 4 (D) Only 5
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B
C
D

A protein D is encoded by a gene which is 5 KB long and has three Hind III restriction enzyme sites. The first one is 0.5 KB from the transcription start site, the second one is 2.5 KB from the first site and the third one 0.5 KB internal to the stop codon. The second site is polymorphic. In order to find out whether fetal cells contain normal or mutated gene, total genomic DNA from the fetal cells was isolated, completely digested with Hind III, separated in an agarose gel, transferred to membrane and detected by a probe against the region between the second and third restriction site.Which one of the following band patterns will be obtained if the fetal cell is hetero-zygous.
A
B
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D

Given below are the experimental protocols to find out the exact location of repetitive DNA sequence in mitotic chromosome by FISH (Fluorescence in situ hybridization). Which one of the following protocols will give the correct result?(A) Mitotic chromosomes were fixed on the glass slide - > Incubated with biotinylated telomeric DNA -> Denatured -> Incubated with fluorescently labeled avidin -> localization observed under fluorescence microscope(B) Mitotic chromosomes were fixed on the glass slide - > Denatured -> Incubated with FITC labeled unrelated non-repetitive DNA sequence biotinylated telomeric DNA -> Counterstained with propidium iodide -> localization observed under fluorescence microscope.(C) ) Mitotic chromosomes were fixed on the glass slide - > Denatured -> Incubated with biotinylated satellite DNA -> Incubated with fluorescently labeled avidin -> localization observed under fluorescence microscope(D) Mitotic chromosomes were fixed on the glass slide - > Incubated with repetitive DNA sequence binding protein -> Denatured - > FITC labeled antibody against protein -> localization observed under fluorescence microscope
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B
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D

Using FRAP (Fluorescence Recovery After Photo Bleaching) techniques diffusion coefficient of three integral membrane proteins M1, M2 and M3 in a kidney cell is calculated as 1µm/sec, 0.05 µm/sec and 0.005 µm/sec respectively. Considering fluid-mosaic nature of biological membrane and relationship of structural organization of integral membrane protein with diffusion coefficient, which protein(s) have highest number of integral membrane domain?(A)M2 and M3 (B) M2 only(C) M3 only (D) M1 and M3
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B
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D

At 25° values of [O]222, the mean residue ellipticity at 222nm, are -33,000 and -3,000 deg cm2dmol-1 for a polypeptide existing in a a-helical (a) and ß-structure (ß), respectively . If this polypeptide undergoes a two-tate heat produced a->ß transition and a value of [O]222 = -18,000 deg cm2dmol-1 is observed at 60°C , then this observation leads to the conclusion that the a-helix conversion to the ß-structure is –(A)40% (B) 50%(C) 55% (D) 60%
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D

An EEG was recorded and its power spectrum analyses were done in rats with implanted electrode for a long time. The power of EEG waves decreased two months after electrode implantation.This observation may be due to the following:1.Glial cells accumulate surrounding the exposed tip of electrodes.2.Degeneration of neurons occurs surrounding the electrode tips due to metal deposition.3.Coatings of electrodes are destroyed with time.4.The microsocket becomes loose with time.Which one of the following is true?(A)Only 1 (B) 1 and 2(C) Only 3 (D) 3 and 4
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D

Tryptic digest of heptapeptide [built from 3 lysine (K), 2 alanine (A), 1 tyrosine (Y) and 1 phenylalanine (F)] yielded tri and tetrapeptide. Which of the following is the correct sequence of heptapeptide?(A)KAYAKFK (B) YKAAFKK(C) KYKAAKF (D) KYAAKFK
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D

100ml of 0.1 M sodium acetate solution has a pH of 8.90. To this solution 1000µl of 1M acetic acid (pKa = 4.76) of pH 2.80 is added. The pH of this mixture will be –(A)8.90 (B) 4.76(C) 2.80 (D) 5.76
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Choose the correct sequence of events in next generation sequencing technology based whole genome sequencing project –(A) DNA extraction –> shearing –> library preparation –> sequencing –> assembly –> finishing –> annotation –> submission to the Genbank(B) DNA extraction –>library preparation –> sequencing –> assembly –> annotation –> finishing –> submission to the Genbank(C) DNA extraction –> shearing –> adapter ligation –> library amplification –> sequencing –> assembly –> finishing –> annotation –> submission to the Genbank(D) DNA extraction –> shearing –> adapter ligation –> library amplification –> shearing -> sequencing –> finishing –> assembly –>annotation –> submission to the Genbank
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. An investigator discovers a new receptor for a known ligand and wanted to identify the binding partner of the receptor i.e. its coreceptor. The antireceptor antibody is not available but anti – GFP antibody is available. Which one of the following strategies is most likely to identify the co-receptor?(A) The GFP- receptor fusion protein is expressed in a cell line and analyzed by LC-MS/MS(B) The GFP- receptor fusion protein is expressed in a cell line and the cells positive for GFP were sorted out, lysed and run on polyacrylamide gel(C) The GFP – receptor protein is coated on ELISA plate, followed by ELISA with anti GFP antibody.(D) The receptor is cloned as a fusion protein of GFP and expressed in stimulated cells. The immune- precipitated complex obtained by anti- GFP antibody was analysed by LC-MS/MS
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D

Plasmids are self replicating small circular DNA elements in bacterial cells that can be said to have a stable symbiotic existence with the host cell. They often carry genes useful to the host. Which of the following is a potential threat to the evolution and stability of the symbiotic coexistence?(A) ‘Copy- up’ mutations that increase the rate of plasmid replication per host cell cycle.(B) Reversible integration of plasmid DNA into the host DNA (C) Transfer of plasmids to the new cells by conjugation(D) Spontaneous curing of plasmids in a small proportion of host cells
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D

During transgenesis, the location of the genes and their number integrated into the genome of the transgenic animal are random. It is often necessary to determine the copy number of genes and their tissue- specific transcription. The following are the possible methods used for the determination –1. Polymerase Chain Reaction (PCR)2. Southern blot hybridization3. Reverse transcriptase PCR4. Western blotChoose the correct set of combinations – (A)1 and 2 (B) 2 and 3(C) 2 and 4 (D) 1 and 4
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A student wrote following statements regarding the comparison of Restriction Fragment Length Polymorphism (RFLP), Random Amplified Polymorphic DNA (RAPD) , Amplified Fragment Length Polymorphism (AFLP) and Simple Sequence Repeats (SSRs) techniques used for generating molecular marker in plants –1. All these techniques can be used for fingerprinting.2. Detection of allelic variation can be achieved only by RFLP and SSRs.3. Use of radioisotopes is required in RFLP and RAPD only.4. Polymerase Chain Reaction is required for all the techniques.Which of the following combination of above statements is correct?(A)1 and 2 (B) 2 and 3(C) 3 and 4 (D) 4 and 1
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D

In order to clone a eukaryotic gene in pBR322 plasmid vector, the desired DNA fragment was produced by PstI cleavage and incubated with PstI digested pBR322 (PstI cleavage site lies within the ampicillin resistant gene ) and ligated. Mixture of ligated cells was used to transform E.coli and plasmid containing bacteria were selected by their growth in tetracycline containing medium. Which type of plasmid/s will be found?(A) Circular pBR322 plasmid containing the target gene and resistant to only tetracycline(B) Circular pBR322 plasmid containing the target gene and resistant to tetracycline only and recircularised pBR322 plasmid resistant to both ampicillin and tetracycline(C) Circular pBR322 plasmid containing the target gene and resistant to only tetracycline, recircularised pBR322 plasmid resistant to both ampicillin and tetracycline and concatemerized pBR322 resistant to both ampicillin and tetracycline(D) ) Circular pBR322 plasmid containing the target gene and resistant to both ampicillin and tetracycline
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During apoptosis, Phosphatidyl Serine (PS) usually present in the inner leaflet of the plasma membrane flips to the outer membrane. Annexin V is a protein that binds to PS. Using this as a tool, we identify the apoptotic cells from necrotic and normal cell populations by FACS using FITC – tagged Annexin V. Propidium Iodide is used to stain the nucleus which generally identifies necrotic and late apoptotic cells. In which area of the plot you should get early apoptotic cells by FACS analysis?(A)Quadrant I (B) Quadrant II(C) Quadrant III(D) Quadrant IV
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A fluorophore when transferred from Solvent A to Solvent B results in an increase in the number of vibrational states in the ground states without any change in the mean energies of either the ground or excited state. What would be the change seen in the fluorophore’s emission spectrum?(A) An increase in emission intensity(B) An increase in emission bandwidth(C) An increase in emission wavelength(D) A decrease in emission wavelength
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You wish to localize a given gene product at subcellular levels following immunofluorescence staining. Routine microscopy could not resolve whether the gene product is localized inside the nucleus or on the nuclear membrane. Which of the following will resolve this unambiguously?1. Sectioning of cell followed by phase contrast microscopy.2. A simulation of 3D picture following confocal microscopy.3. Optical sectioning and observing each section.4. Freeze fracturing followed by Scanning Electron Microscopy.(A)1 and 2 (B) 2 and 3(C) 3 and 4 (D) 1 and 3
A
B
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D

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