Digestive system_intestine

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Session Objectives : Session Objectives Digestion in Stomach Digestion in Small Intestine Accessory Glands Absorption of digested food Egestion

The Digestive System of man : The Digestive System of man Alimentary canal andaccessory glands

Histology of alimentary canal : Histology of alimentary canal

Slide 6 : All the four layers show modifications in different parts of the alimentary canal In duodenum, glands are present in sub-mucosa Mucosal epithelium has goblet cells which secrete mucus that help in lubrication. Mucosa also forms glands in the stomach (gastric glands) and crypts in between the bases of villi in the intestine (crypts of Lieberkuhn). Histology of alimentary canal

Stomach : Stomach C-shaped sac located at left side in upper portion of abdominal cavity. It has three regions

Glands in Stomach : Glands in Stomach

Specialized cells in stomach : Specialized cells in stomach

Gastric Glands : Gastric Glands Secrete gastric juice: Goblet cells: mucus. Parietal cells: HCl and intrinsic factor. Chief cells: pepsinogen. Enterochromaffin-like cells (ECL): histamine and serotonin. G cells: gastrin. D cells: somatostatin. Stomach: ghrelin. Feeling Hungry ? ghrelin functions to increase hunger though its action on hypothalamic feeding centers

Functions of stomach : Functions of stomach Mixes food with gastric juice( Mucous, HCl, Pepsinogen, gastrin) Acts as a churn for foodand liquefies it into chyme Retains food giving enzymes time to act Digests proteins through action of enzymes Performs some absorption of salts and alcohol Produces gastric intrinsic factor (GIF) – Helps in absorption of vitamin B12

Gastric Digestion : Protein containing food stimulate secretion of gastrin from pyloric gland Gastrin stimulate gastric gland for the secretion of HCl, pepsinogen and mucus Pepsinogen in acidic medicum forms pepsin which digest proteins Small quantities of other gastric juice is also secreted – gastric lipase, gastric amylase, gelatinase Enterogasterone regulates the flow of chyme into intestine Gastric Digestion

Gastric juice : Gastric juice

Illustrative problem : Illustrative problem What are stomach ulcers? An ulcer is an irritation in the gastric mucosa through which gastric juices digest the stomach wall. Solution : What is achlorhydria ? It leads to pernicious anemia. How ?

Slide 15 : What is achlorhydria ? It leads to pernicious anemia. How ? When acid producing parietal cells of the stomach are destroyed, which frequently occurs in chronic gastritis, the person develops achlorhydria (lack of acid secretion). It leads to pernicious anemia because of the failure of the secretion of GIF leading to failure of maturation of the red blood cells in the absence of vitamin B12 stimulation of the bone marrow.

Structure of intestine : Structure of intestine Duodenum Jejunum Ileum

The small intestine - Duodenum : The small intestine - Duodenum C-shaped structure (about 25cm long) Most fixed part of the intestine Begins at pyloric sphincter and ends into jejunum Bile and pancreatic ducts open into it Possesses Brunner’s glands (help neutralize stomach acidity) Pancreas lies within its loop

Digestion in small intestine : Digestion in small intestine Three basic stimuli required for Pancreatic secretion :- Acetylcholine : released from parasympathetic vagus nerve and stimulates acinar cell of pancreas Cholecystokinin : released from duodenal mucosa and stimulates acinar cell of pancreas and liver for bile secretion Secretin : released from duodenal mucosa and stimulates secretion of sodium bicarbonate for neutralizing the acidic properties of chyme

Pancreas : Pancreas Acinar cells produce – Pancreatic juice – Sodium bicarbonate – Pancreatic amylase Carboxypeptidase Endopeptidase Lipase Trypsin chymotrypsin

Digestion in small intestine : Digestion in small intestine Proteoses and peptones of chyme excites cholecystokinin which in turn stimulate pancreas for enzymatic secretion Meanwhile secretin get excited by low pH (<4.5 – 5) and secretes bicarbonate thereby medium becomes basic (pH 8) Proteolytic pancreatic enzymes are synthesized in the inactive forms like trypsinogen, chymotrypsinogen and procarboxypolypeptidases

Digestion in small intestine : Digestion in small intestine Trypsin becomes active by an enzyme called enterokinase secreted by intestinal mucosa. Trypsinogen can be autocatalytically activated by trypsin chymotrypsinogen and procarboxypolypeptidases are activated by trypsin Trysin and chymotrysin are endopeptidases. They split partially digested proteins into peptides of various size but do not causes release of amino acids Carboxypolypeptiddases is exopeptidases. It splits peptides into individual amino acid

Digestion in small intestine : Digestion in small intestine Digestive enzyme for carbohydratae is pancreatic amylase. It hydrolyzes starches, glycogen and other carbohydrate (except cellulose) into disacchrides Enzymes for fat digestion are pancreatic lipase, chloesterol esterase and phospholipase. Fatty acids and monoglycerides are formed

Bile : Bile Chloecystokin stimulates bile secretion from Gallbladder. Bile consists of bile salts, chloesterol, lecithin and electrolytes of plasma. Bile plays an important role in fat digestion. It emulsify large fat into minute particle for the action of enzyme lipase. It also aid in absorption of digested end product of fat by intestinal mucosa. Do You Know ? Bile serves as means of excretion for several waste product from blood. These include especially bilrubin, an end product of haemoglobin destruction and excess of chloesterol

Illustrative Problem : carbohydrates fats proteins All of these If the pancreas is removed, the compound which remains undigested is Illustrative Problem

Intestinal villi : Intestinal villi Adaptations of villi for absorption Large surface area for absorption Thin walled for easy passage of nutrients Rich supply of blood capillariesand lacteals

Specialized cells in the intestine : Specialized cells in the intestine Crypt’s of Leiberkuhn (intestinal glands)-tubular glands possessing various specialized cells. It lie between intestinal vili. Intestinal surface of crypts and vili are covered by epithelium consisting of two types of cell – Goblet and enterocytes Goblet cells-secrete mucous for lubrication and protection of alimentary canal. Enterocytes – It forms intestinal secretion and helps in digestion and absorption of nutrients, processing of fats, transport of small nutrients.

Specialized cells in the intestine : Specialized cells in the intestine Enterocytes of mucosa covering vili contain digestive enzymes Several peptidases for splitting small peptides into amino acids Four enzymes for splitting disacchrides inot monosacchride – sucrase, maltase, isomaltase and lactase Small amount of intestinal lipase for digestion of fats

The large intestine : The large intestine

The large intestine : The large intestine Extends from ileocaecal junction to anus (about 1.5m long) The surface bears longitudinal bands of muscles called taenia coli It bears sacculations (called haustra) which are a characteristic feature of large intestine It is divided into the caecum, colon, rectum and anus.

The Large intestine : The Large intestine Caecum Blind ending sac between the ileum and colon The opening of ileum into the caecum is guarded by the iliocolic valve It is functional in herbivores, but has lost its importance in man The vermiform appendix is attached to the caecum It is an elongated (10 cm) projection made up of lymphoid tissue

The Large intestine : The Large intestine Colon - (upto 5ft long) distinguished into Ascending colon- reabsorbs useful materials Distal colon (transverse, descending and sigmoid) excretes wastes Absorbs large amounts of water Reabsorbes bile salts and electrolytes Powerful storage and waste disposal site in the body Stores wastes till excretion Through involuntary contractions pushes wastes to anus. Functions

Functions of the Large Intestine : Functions of the Large Intestine Water absorption Reabsorption of salts and electrolytes Fermentation – to breakdown the dietary fibres with the help of intestinal bacteria- Lactobacillus acidophilus, E.coli, Klebsiella Bacterial fermentation produces- Short chain fatty acids Protein metabolites Gases (methane, ammonia and hydrogen sulphide)

Illustrative problem : Illustrative problem What do the intestinal bacteria do ? Solution: They mainly have a protective role. By inhabiting the large intestine they keep out bacteria that may cause disease. They take up all the available nutrients and space and crowd out dangerous competitors. Some also synthesize vitamins K and B12. They do not contribute much to digestion but form bulk of the faeces (constitute 20 – 50%) of the dry weight of feces).

Diarrhea : Diarrhea Whenever a segment of large intestine is irritated , the mucosa secretes large amount of water and electrolyte. This acts to dilute the irritating factors and cause to rapid movement of feces towards anus. The result is diarrhea.

Digestion of nutrients : Digestion of nutrients

Accessory glands : Accessory glands Liver Gall bladder Pancreas

Accessory glands– The Liver : Accessory glands– The Liver For digestion produces bile Stores glucose as glycogen Processes fats and amino acids Stores some vitamins Detoxifies poisons and drugs(clearing house) Makes blood proteins

Accessory Gland - The Gall Bladder : Accessory Gland - The Gall Bladder Small pear shaped organ (3-4 inches long) It is connected to liver by the cystic duct It stores and concentrates bile Opens into the duodenum through the bile duct Bile Is produced from cholesterol in the liver It emulsifies fats and neutralizes acids in partly digested food. Bile salts – Glycocholic acid, taurocholic acid.

Absorption and Assimilation : Absorption and Assimilation Digestion products are absorbed through micro-vili of small intestine Fatty acids, monoglycerrides, chlesterol and other fat soluble substances are absorbed by simple diffusion through brush border of the cells lining the inestinal vili Water soluble substances (alcohol) are absorbed by osmosis Hydrophilic lipid insoluble sugar like fructose are absorbed by facilitated diffusion. It occurs with the help of specific transporter protein The process is powered by coupling sugar transport to electrochemical gradient for Na+ across the brush border.

Model of Glucose Absorption : Model of Glucose Absorption

Absorption and Assimilation : Absorption and Assimilation Amino acids are absorbed by active transport Fat soluble but water insoluble monoglycerides, fatty acids and glycerol are first incorporated into water soluble droplets called micelles. From these micelles fatty acids, glycerol, sterols and fat soluble vitamins are absorbed by facilitated diffusion and are reconstructed within the absorptive cells (smooth ER) into triglycerides Remnant of micelle is left behind and combine with new fatty acids and monoacylglycerols Reconstructed triglycerides later combine with phospholipid and chloesterol and release into lymph in the form of protein coated water soluble fat droplets called chylomicrons. These droplets are transported to blood through lymphatic system

Absorption of Fats : Absorption of Fats

Routes of absorbed nutrients : Routes of absorbed nutrients Water soluble substances Fat soluble substances

Slide 44 : Fate of absorbed nutrients Glucose To release energy Excess glucose is converted to glycogen and stored in liver and muscles Excess glucose is converted into fat for storage Fats As energy reserve Stored beneath skin and around internal organs Amino acids Build up body tissues, production of hormones, enzymes and antibodies Replace worn out tissues Excess amino acids are deaminated in the liver to form urea.

Egestion : Egestion Peristalsis pushes the slurry of indigestible materials of small intestine into large intestine or colon The colon absorbs most of the water, electrolytes and ions Active pumping of Na+ and water ffrom chyme Bacteria in colon produces vit B12, K, thiamine and riboflavin that are absorbed by the wall of colon

Fill in the blanks : : Fill in the blanks : The gland found in submucosa of deodenum is………….. Vili is a ………………… layer of alimentary canal GIF is secreted by………………cell of stomach Serotonin is secreted in …………….. Gastrin is secreted in …………… Enterokinase is secreted by…………. The tubular branched exocrine gland situated in submucosa of alimentary canal is………. Argentaffine cells secretes……………… Flow of chyme from stomach is regulated by ------- Chylomicrons are formed by triglycerides & …………

Answer: : Answer: The gland found in submucosa of deodenum is Bruner’s gland Vili is a mucosal layer of alimentary canal GIF is secreted by parietal cell of stomach Serotonin is secreted in stomach Gastrin is secreted in stomach Enterokinase is secreted by deodenum The tubular branched exocrine gland situated in submucosa of alimentary canal isBruner’s gland Argentaffine cells secretes serotonin & histamine Flow of chyme from stomach is regulated by enterogasterone Chylomicrons are formed by triglycerides & proteins

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