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General principles of pharmacology : General principles of pharmacology

Objectives : Objectives By the end of this topic you should be able to: Define the main terms used in pharmacology Explain the factors that influence drug movement across membranes Describe the key features of each of the four phases of pharmacokinetics Explain the time course of responses to a drug Explain why an understanding of half-life is important to drug doseing

Pharmacology - terminology : Pharmacology - terminology Drug - chemical that acts on a living process Pharmacology - study of interaction between drugs and living organisms Therapeutics - drugs used to diagnose, prevent and treat disease or prevent pregnancy Pharmacopoeia - list of all authorised drugs Toxicology - study of poisons

What are the features of an ideal drug? : What are the features of an ideal drug?

The ideal drug : The ideal drug What are features of an ideal drug?

Drug formulations : Drug formulations Tablets & capsules convenient and commonest dosage forms but likely to be no good if the drug cannot be absorbed in the gastrointestinal tract or if the patient (e.g. a child) cannot swallow them or is unconscious

Drug formulations : Drug formulations Injections & infusions rapid action but impractical for treating chronic (long term) illnesses can deliver large volumes of fluids there may be a danger of local tissue damage from toxic agents

Drug formulations : Drug formulations Pessaries & suppositories can deliver the drug to local area where required but have limited general use made with vehicle (eg cocoa butter) that melts at body temperature storage - in fridge

Drug formulations : Drug formulations Solutions, suspensions & elixirs useful for children and the elderly but are bulky and less useful if the drug is unpalatable or unstable in the presence of water Aerosols & dry powder inhalations good for drugs required in the lungs but can be difficult to administer the dose correctly

Drug formulations : Drug formulations Ointments, creams & paints topical application Transdermal patches convenient if the dose needs to be released over a long period (e.g. hormone replacement therapy) but can cause irritation

Drug Combinations : Drug Combinations Drugs are rarely administered alone - often they are combined in a formulation with other medical and non-medical agents this can confer special physical or chemical properties E.g. protect from gastric pH E.g. fast acting analgesics

Drug names : Drug names Trade or brand name Antenex, Valium, Ducene Generic or non-proprietary name diazepam Chemical name 7-chloro-1,3-dihydro-1-5-phenyl-2H-1,4-benzodiazepin-2-one

Drug names – another example : Drug names – another example Trade or brand name Plavix Generic or non-proprietary name Clopidogrel hydrogen sulfate Chemical name methyl (+)-(S)-?-(2-chlorophenyl)-6,7-dihydrothienol[3,2-c] pyridine-5(4H)-acetate sulfate

Slide 14 :

Principles of pharmacology : Principles of pharmacology Pharmacokinetics absorption distribution metabolism excretion Pharmacodynamics how does the drug work- biochemical and physiological action of drugs & how they produce these effects

What factors influence drug movement across membranes? : What factors influence drug movement across membranes? molecule size - little effect, most drugs are small lipid solubility - nonpolar molecules move across membranes more rapidly than polar ones ionisation – unionised molecules can cross membranes ionised drugs use transport systems to move drugs from gut to blood and from blood to urine

Ionisation : Ionisation Strong acids & bases are ionised at all pHs Most drugs are weak acids or bases and are ionised at physiological pH (usually pH 7.4) Degree of ionisation depends on the pH of the solution e.g. stomach, urine

Ionisation of weak acids & bases : Ionisation of weak acids & bases

For a weak acid : For a weak acid

For a weak base : For a weak base

In summary: : In summary: Acids tend to ionise in alkaline media ? acidic drugs tend to accumulate on the alkaline side Bases tend to ionise in acidic media ? basic drugs will accumulate on the acidic side

A question : A question Why would people who want to increase the amount of amphetamine in the body take bicarbonate to make the urine more alkaline? Alkaline urine increases the proportion of amphetamine reabsorbed from the urine into the blood acidic urine: 78% lost in 24 h alkaline urine: 45% lost in 24 h

Pharmacokinetics : Pharmacokinetics

Absorption : Absorption Movement of drug from site of administration to the blood absorption influenced by rate of dissolution (How fast does it dissolve?) surface area of membrane most drugs (~80%) are administered orally

Oral administration : Oral administration What factors influence absorption of drugs following oral administration?

First pass metabolism : First pass metabolism After a drug is absorbed from GIT, it is delivered to the liver via the portal circulation Orally administered drugs metabolised in the liver therefore undergo first pass hepatic metabolism From: http://www.nature.com/nrd/journal/v2/n3/box/nrd1032_BX3.html

Parenteral administration : Parenteral administration intravenous injections & infusions intramuscular injections subcutaneous injections inhalation sublingual topical rectal other specialised routes eg into joints or spine

Bioavailablity : Bioavailablity The amount of drug that actually reaches the systemic circulation as a % of the administered dose IV administration = 100% Oral & other routes - varies

Distribution : Distribution distribution is influenced by 3 factors: blood flow how much drug actually reaches target site ability to leave blood only free (i.e. unbound drug can leave blood) special case: blood-CSF barrier is only permeable to lipid soluble drugs ability to enter target organ

Drug-protein complexes : Drugs, like other body chemicals, can be bound reversibly to proteins (eg albumin) Drug-protein complexes From: Lehne ( 2002) Pharmacology for Nursing Care

% Protein binding [interest only] : % Protein binding [interest only]

Metabolism : Metabolism Metabolism occurs predominantly in the liver Other sites – plasma, GIT, lungs 2 main types of biochemical processes – Phase I and Phase II Phase I – catabolic reactions Tends to make more chemically reactive products Uses cytochrome (CYP) P450 family of enzymes Phase II – anabolic (synthetic) reactions Conjugation reactions that tend to result in inactive products

Consequences of metabolism : Consequences of metabolism makes the drug more water soluble to increase renal excretion pentobarbital ? pentobarbital alcohol

Slide 34 : Inactivates the drug procaine ? PABA Increase or change the action codeine ? morphine Activate a prodrug Levodopa ? dopamine Alter toxicity paracetamol ? hepatotoxic compound

Special considerations in metabolism of drugs : Special considerations in metabolism of drugs Age infants and elderly Induction of drug-metabolising enzymes accelerates drug metabolism First pass effect Hepatic inactivation of oral drugs Nutritional status deficiency of co-factors used in metabolism Competition between drugs If they use the same metabolic pathway can reduce metabolism of one or both drugs

Renal excretion : Renal excretion Steps in renal excretion: glomerular filtration - all free, small drugs are filtered passive reabsorption - lipid soluble drugs can pass back into blood & are difficult to excrete active secretion - 'pumps' compounds from blood to urine. Competition for transporter can slow excretion e.g. probenacid slows penicillin excretion

Factors affecting renal excretion : Factors affecting renal excretion pH dependent ionisation Why might aspirin overdose treatment involve making the urine more alkaline? Competition for active tubular resorption E.g. penicillin excretion can be delayed by concomitant excretion of probenecid Age New borns have immature kidneys

Non-renal excretion : Non-renal excretion Breast milk lipid soluble drugs can enter milk may affect infant Bile reabsorption from bile termed enterohepatic recirculation Eg digoxin and morphine Lungs for volatile compounds Sweat, tears, faeces minor & not therapeutically important:

Summary : Summary Note central role of liver and kidney in these processes

Time course of responses : Time course of responses once administered, a drug enters the blood and reaches peak concentration, then the concentration decreases again as it is cleared (metabolised and excreted) the faster the clearance the less time it will remain in body determines how frequently a drug needs to be given

Slide 41 :

Half-life (t½) : Half-life (t½) Since the concentration of drug at the site of metabolism and excretion determines the amount cleared, the clearance rate is often expressed as the half-life Half life = time taken to reduce concentration of drug by 50% may be altered by age, weight, liver or kidney disease, drug interactions useful for calculating intervals for repeat doses some drugs don't have a half-life - a constant amount is excreted per unit time e.g. alcohol

Slide 43 :

Slide 44 : From: Lehne ( 2002) Pharmacology for Nursing Care

Half-life : Half-life Different drugs in a therapeutic class may have different half-lives. Example: hypnotics short half-life used for those who have difficulty falling a sleep longer half-life used for those with early morning insomnia Sustained release preparations artificially increase half-life Drugs with long half-lives can be problematic – why?

Repeat doses : Repeat doses By giving repeated doses at particular time intervals, administration keeps up with clearance and a steady concentration can be achieved

Slide 47 : Fluctuations in plasma concentration can be reduced by: infusions decrease dose and increase frequency Plateau is normally reached after 4-5 half-lives problem if half-life is long e.g. diazepam, takes a long time to reach therapeutic doses can give ‘bolus’ dose - often a double dose to reach plateau more rapidly, or give injection then take orally can be a problem as adverse effects more common with higher dose

Slide 48 : Same dose (4 units) is given at each t½ (1 h) After 4-5 t½ there is little variation between the min and max concentrations From: http://cpharm.vetmed.vt.edu/VM8314/kineticsnotes2001WithPics.htm

Slide 49 :

Therapeutic index : Therapeutic index Large therapeutic index = safer drug If a drug has a large therapeutic index, there is little chance of accidentally reaching toxic concentrations Eg penicillin If drug has a small therapeutic index, then plasma levels must be monitored closely to prevent toxic concentrations Eg gentamicin, lithium, digoxin

Slide 51 : From: Lehne ( 2002) Pharmacology for Nursing Care

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