MCQs for Agrobacterium mediated gene transfer in plants

MCQ FOR AGROBACTERIUM MEDIATED GENE TRANSFER(SOLVED) 1. Ti plasmid are used to create transgenic ______. A Plants B Animals C Fungi D All of the above 2. The Ti plasmid is obtained from which species? A Escherichia coli B Agrobacterium tumifaciens C Thermus aquaticus D Bacillus thuriengiensis 3. Which vector would you chose to express a foreign gene in a plant? A Baculovirus vector. B Retroviral vector. C Yep vector. D Ti vector having T-DNA 4. pBIN 19 binary vector consist of A lac z' gene B Kanamycin resistance gene C Cloning sites D All of above 5. Which one is not used in making genetically modified crops ? A Vector based on plant Virus B Various plasmid DNA C Artificial plasmid of animals D Natural plasmid of Agrobacterium 6. Which is not a method of DNA insertion A Transformation B Transfection C Microinjection D None 7. Agrobacterium tumifaciens causes A Rust B Mildew C Crown Gall disease D Hairy root disease 8. Plant cells can easily regenerate into entire plant because A These are totipotent B Embryo is totipotent C Stem cells are pluripotent D They divide actively 9. Which of the following microorganisms has been widely used as a vehicle for foreign gene transfer in plants ? A Agrobacterium B Escherichia coli C Bacillus anthracis D TMV 10. Ti plasmid is present in A Agrobacterium B Cymbidium C Dendrobium D Syzigium 11. The first transgenic crop produced was A Pea B Flax C Tobacco D Cotton 12. Insect tolerant gene from Bacillus thurngiensis is introduced into plants using plasmid of A Escherichia coli B Agrobacterium rhizogenes C Haemophilus influenza D Arabidiopsis thaliana 13.Agrobacterium tumifaciens is an effective vector for use with A Corn B Rice C Wheat D Soyabean 14. What roles do opines play in crown gall diseases ? A Source of Carbon, Nitrogen and energy for the Agrobacterium B Transfer of T-DNA to plant cells C Attachment of Agrobacterium to the plants D Induction of expression of Vir genes 15. A plant Genetic Engineer wishes to transfer and express a gene from Sunflower into beans,which of the following would be vector of choice ? A lambda phage B pBR322 C Ti plasmid D Maize streak virus 16. Golden Rice is a transgenic crop with a trait for A Pest Resistance B High vitamin A content C Drought Resistance D High protein content 17. Transfer of T-DNA from Ti plasmid into plant cell is mediated by A mob genes B vir genes C nif genes D octapine genes 1