CBSE Biotechnology sample paper class XII 2009

http://www.cbseguess.com/-------------------------------------------------------------------------------------------------------www.cbseguess.com Other Educational Portals www.icseguess.com | www.ignouguess.com | www.dulife.com | www.magicsense.com 1 Sample Paper – 2009 Class – XII Subject – BIOTEHNOLOGY Time: 3 hrs Max Marks: 70 SECTION A 1. Why cannot E. coli be used to overproduce penicillin? 2. What is the role of phenol red in animal cell culture? 3. Expand FGF & PDGF. 4. A soil microorganism produces novel metabolites in nanometer concentration (nm). Suggest a way to increase its production in quantities that are economically viable? 5. In isolating recombinant insulin from a culture of E. coli the cells were filtered & the filtered was subjected to a purification protocol. However no insulin was obtained. Why? SECTION B 6. Explain why Bt cotton flowers undergo pollination by butterflies & bees in spite of being insect/pest resistant? 7. if you wish to scale up cells derived fro human tissue, what kind of culture set up will you use and why? 8. Name any four physical/chemical properties of enzyme which might be useful to change by site directed mutagenesis. Support your answer by an engineered protein/enzyme. http://www.cbseguess.com/-------------------------------------------------------------------------------------------------------www.cbseguess.com Other Educational Portals www.icseguess.com | www.ignouguess.com | www.dulife.com | www.magicsense.com 2 9. By what factors the stability of the protein can be maximize during its purification? 10. An experimental technique allows purified mRNA from a eukaryotic cell o be hybridized (paired up) with the DNA which codes for it. Under he electron microscope the following structure is observed. 11. Explain random shotgun approach of sequencing. 12. A. name the phenomenon represented by the highlighted difference between the two polynucleotide from two different DNA. B. Interpret the given SNP map and explain how this can be used to predict medicine response? http://www.cbseguess.com/-------------------------------------------------------------------------------------------------------www.cbseguess.com Other Educational Portals www.icseguess.com | www.ignouguess.com | www.dulife.com | www.magicsense.com 3 13. a. Label parts a, b, c and d. b. What is the role of part d? 14. Define chemostat & turbidostat. 15. AN E. coli cell produces at least 2000 different proteins. One of these is our enzyme of interest produced a eve of 3000 molecules pr cell under optimum condition. If we have to purify 1 gram of this intracellular enzyme, estimate how many cells of bacteria will be required theoretically? I is given that the molecular weight of e enzyme of interest is 1, 00,000 Daltons. SECTION C http://www.cbseguess.com/-------------------------------------------------------------------------------------------------------www.cbseguess.com Other Educational Portals www.icseguess.com | www.ignouguess.com | www.dulife.com | www.magicsense.com 4 16. What are the two advantages of cDNA library over genomic library? What is the role of Mg2+ ion in PCR? 17. Draw a neat labeled diagram of MS. If a protein is having molecular weight 300000 Dalton and charge on that is 3 H+, then at what m/z ratio doe this protein would be detect out? 18. Explain briefly Proteomics. 19. Explain three methods of identification of host containing foreign gene. 20. Explain how DNA Microarray technique to study cellular responses to environment? 21. What kinds of analysis ca e made using various bioinformatics tools? 22. How the aeration and mixing achieved in the laboratory? Recombinant insulin is produced at 100 mg/l by E. coli at a cell concentration of 1 g/l. calculate the volume of fermenter needed for producing 1 kilogram of insulin when the cell concentration is 50 g/l and insulin production is 200 mg/g of cells. 23. What are the uses of callus and protoplast culture? 24. What are the advantages of using established cell lines? What are the special features of ICM cells? 25. What is the main difference between pluripotential, multipotential and unipotential Cells? OR Explain ES cell cure. How chimeric mouse can be produced? SECTION D 26. a) What is edible vaccine? What are heir advantages? b) How the unnecessary pollination can be prevented using transgenic plant? c) How the insect resistant plants can be produced? d) Define biolistic. 27. Explain two dimensional gel electrophoresis? What are advantages of ESI over MALDI? OR Explain structure function relationship with taking an example of chymotrypsin. How would you demonstrate that given active protein is having active serine? 28. a) In the following diagram what do the A, B & C represent? http://www.cbseguess.com/-------------------------------------------------------------------------------------------------------www.cbseguess.com Other Educational Portals www.icseguess.com | www.ignouguess.com | www.dulife.com | www.magicsense.com 5 b) Why the Agrobacterium tumefaciens called as natural genetic engineer of plants? c) Write various markers used in section/screening of transgenic plants. OR Write on the bioethics of plant genetic engineering. KENDRIYA VIDYALAYA NO. 3 DELHI CANTT. Paper Submitted by: payal priyadarshani Email :-payal0908@gmail.com