CBSE Biotechnology sample paper3 class XII 2009

http://www.cbseguess.com/-------------------------------------------------------------------------------------------------------www.cbseguess.com Other Educational Portals www.icseguess.com | www.ignouguess.com | www.dulife.com | www.magicsense.com Guess Paper – 2009 Class – XII Subject – Biotechnology Time: 3Hrs Max. Marks: 70 General Instructions i. All questions are compulsory. ii. There is no overall choice. However, an internal choice has been provided in one question of 3marks and three questions of 5marks. You have to attempt only one of the choices in such questions. Question Paper contains four sections – A, B, C and D. iii. Question numbers 1 to 5 are very short answer questions, carrying 1 mark each. iv. Question numbers 6 to 15 are short answer questions, carrying 2 marks each. v. Question numbers 16 to 25 are also short answer questions, carrying 3 marks each. vi. Question numbers 26 to 28 are long answer questions, carrying 5 marks each. SECTION-A 1. Name the source organism of subtilisin. 2. Name the chemical which is used to coat the artificial seeds. 3. Name the single letter IUPAC code for Pyrimidine and Purine? 4. What will you call bacteria which are not able of use Biotin on minimal medium? 5. What is the idea behind using thermostable Taq polymerase in PCR instead of normal polymerase? SECTION-B 6. Name the first sequenced and who developed the method of sequencing. 7. What are (RE) restriction end nucleases? Why are they called as molecular scissors? 8. Expand and define PCR? Write its main steps? 10. Draw a schematic diagram to isolate recombinant insulin. 11. Explain the method of producing distant hybrids for desired agronomic traits 12. T cells reject transplants. How is kidney transplantation successfully done? 13. Name the bacteria who is called natural genetic engineer and why? Explain. 14. Name and explain different non covalent forces involved in the structural organization of proteins. 15. What are cDNA library? Write its merits over Genomic library in rDNA technology. http://www.cbseguess.com/-------------------------------------------------------------------------------------------------------www.cbseguess.com Other Educational Portals www.icseguess.com | www.ignouguess.com | www.dulife.com | www.magicsense.com SECTION-C 16. Explain DNA Microarray technology? Write its main applications 17. What is ELISA? Mention the main steps of this technique. 18. Mention three methods of preserving microbial strains. 19. What is the principle of MALDI-TOF? What is its main use in protein studies? 20. What are data retrieval tools? Write entrez map showing the hard link relationship between databases? 21. What are the principles between isoelectric focussing & SDS PAGE technique? Why is two dimensional electrophoresis better than one dimensional electrophoresis? 22. Define the following: a. Bioremediation. b. Cosmids c. Database. 23. What is site directed mutagenesis? Indicate its application? 24. Determine the no. of generation and generation time of a bacterial culture containing 100 cells increased its population to one billion in 10 hours. 25. What is micro projectile bombardment? Write its significance in vector less gene delivery process? SECTION-D 26. What do you mean by structure function relationship in proteins? Illustrate your answer by the example of chymotrypsin or sickle cell anemia? 27. What are vectors? Give an illustrated account of different bacterial plasmid as cloning vectors? 28. What is gene prediction & gene counting? Write gene prediction algorithms. OR Write short note on (a) Nick translation b. SNPs. Or FISH Paper Submitted by: Dinesh Email: drdcs@rediffmail.com Phone No. 9826533885