CBSE Biotechnology sample paper2 class XII 2008

http://www.cbseguess.com/-------------------------------------------------------------------------------------------------------www.cbseguess.com Other Educational Portals www.icseguess.com | www.ignouguess.com | www.dulife.com | www.magicsense.com Sample Paper -2008 Class -XII Subject -Biotechnology Time: 3 Hrs M.M. 70 General Instructions ( Read them carefully) a. This question paper consist of four sections A, B, C and D Section A contains5 questions of 1 mark each. Section B contains10 questions of 2 marks each. Section C contains10 questions of 3 marks each and Section D contains 3 questions of 5 marks each. b. All questions are compulsory c. There is no overall choice. However internal choice has been provided one question of 3 marks and two questions of 5marks. Attempt only one of the choices in such questions. d. Question number 1 to 5 are to be answered in one word or one sentence each e. Question numbers 6 to 15 are to be answered in approximately in 20-30 words each. f. Question numbers 16 to 25 are to be answered in approximately in 30-50 words each g. Question numbers 26 to 28 are to be answered in approximately in 80-120 words each. Section: A 1. Write a chemical equation for the production of products from reactants or raw material. 2. What decides the length of DNA strand in Sanger’s chain termination method? 3. Name any two enzymes which are used in protoplast culture. 4. Write the bases which are denoted by symbol D. 5. Name the chemical which is used to coat the artificial seeds and its use? Section: B 6. Name the database which was created to manage redundancy in ESTs. 7. Distinguish between cDNA library and Genomic DNA library. Why cDNA is preferred over genomic DNA in molecular researches? 8. Define the term proteomics. Write the importance of expressional proteomics and functional proteomics. http://www.cbseguess.com/-------------------------------------------------------------------------------------------------------www.cbseguess.com Other Educational Portals www.icseguess.com | www.ignouguess.com | www.dulife.com | www.magicsense.com 9. Write the microbial source and the restriction sites of Bam HI and Hind III enzymes. 10. Why is it necessary to use high voltage in electroporation process? 11. Why are lyophilized microbial cultures viable for several years? 12. Write the functions of t-PA and Erythropoietin. 13. What do you mean by MCS ? Write its other name and mention its advantage. 14. What are edible vaccines? Write their advantage over traditional vaccines? 15. Name an interspecific somatic hybrid and intergeneric somatic hybrid each and who developed them? Section: C 16. Name any three techniques which are used to determine the purity of proteins. 17. Explain the principle and structure of Mass Spectrometer in proteomics. 18. What are plasmids? Name few earliest plasmid vectors? Distinguish between shuttle and expression vectors. 19. A recombinant protein is produced at 100 mg/ml by Bacillus subtilis at a cell concentration of 1g/L. Calculate the size of the fermenter required to produce 500 gram of recombinant protein when the cell mass concentration of 1g/L and protein production is 100 mg /gm of cell 20. What do you mean by strain improvement? Explain it by giving an example. 21. Explain the strategies adopted to produce transgenic plants with following traits. (a) Biodegradable plastics (b) Increased nutrient quality (C) Insect resistance Or Mention the social and ethical problems associated with plant genetic engineering? Write their remedies also. 22. What are culture collection centers? Write their advantages? Name one microbial culture collection center in India. 23. What is embryonic stem cell culture? Write their characteristics with special emphasis in creation of chimeric mouse. http://www.cbseguess.com/-------------------------------------------------------------------------------------------------------www.cbseguess.com Other Educational Portals www.icseguess.com | www.ignouguess.com | www.dulife.com | www.magicsense.com 24. Differentiate between monoclonal antibodies and polyclonal antibodies? Name and explain the technology to produce monoclonal antibodies, commercially. 25. Compare different types of microbial culture in a tabulated form... Section: D 26. What is DNA library? Name its main types. Explain all the steps and draw diagrams involved in making a gene library. 27. Name and explain a method with its principle, major steps to compare the gene expression in breast cancer cell and a normal cell. Or What is PCR? Write its uses? What is its principle? Name the steps and draw the diagrams involved in it. 28. Write the significance of Genomic Sequencing Projects. Name and explain the main methods used in small scale sequencing of entire genomes. *****************************