Teaches following Subjects/Exams
Principles of Genetics (Bachelor of Science: Microbiology)
Language of Instruction:
English
Education
B.Sc Biotechnology
Banasthali Vidyapith, India
Jun 2006 - Jun 2008
•One International research paper in INCOB 2008,Taipei .
Publications and Research
Predicted metal binding sites for phytoremediation
Ashok Sharma*, Sudeep Roy, Kumar Parijat Tripathi, Pratibha Roy, Manoj Mishra, Feroz Khan, Abha Meen
Metal ion binding domains are found in proteins that mediate transport, buffering or detoxification of metal ions. The objective of the study is to
design and analyze metal binding motifs against the genes involved in phytoremediation. This is being done on the basis of certain pre-requisite
amino-acid residues known to bind metal ions/metal complexes in medicinal and aromatic plants (MAP’s). Earlier work on MAP’s have shown
that heavy metals accumulated by aromatic and medicinal plants do not appear in the essential oil and that some of these species are able to grow
in metal contaminated sites. A pattern search against the UniProtKB/Swiss-Prot and UniProtKB/TrEMBL databases yielded true positives in
each case showing the high specificity of the motifs designed for the ions of nickel, lead, molybdenum, manganese, cadmium, zinc, iron, cobalt
and xenobiotic compounds. Motifs were also studied against PDB structures. Results of the study suggested the presence of binding sites on the
surface of protein molecules involved. PDB structures of proteins were finally predicted for the binding sites functionality in their respective
phytoremediation usage. This was further validated through CASTp server to study its physico-chemical properties. Bioinformatics implications
would help in designing strategy for developing transgenic plants with increased metal binding capacity. These metal binding factors can be used
to restrict metal update by plants. This helps in reducing the possibility of metal movement into the food chain.